FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5916787
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA16850.015.9784581
GACCGAG101000.015.37079769
ACCGAGG86800.015.18269669
CCGAGGC62800.013.7336469
TATCCCG8900.013.5697365
TATACCG8250.013.3841065
TAGAGCG11100.013.367155
GTATAGG7500.013.3467931
TATACTG16050.013.3293835
CTAGGAC7850.013.1869693
GTATTAG10800.013.1038921
TATAGGG5900.012.8675862
CTATACT12150.012.7799634
GGCTGAT35100.012.77737169
CGAGGCT49800.012.67730868
AGACCGA129100.012.61307669
CCCCCCA10300.012.3927059
TAGGACA11000.012.233914
TATAGAG13000.012.2107092
ACCCCCC9650.012.154958
Produced by FastQC (version 0.11.2)