FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351163
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGA501.8574417E-434.5042722
ATCCCGG450.00461556730.6704656
CCGGGAT450.00461878430.666143
GCTATTA703.8290542E-529.587731
GTAATAT605.410707E-428.7535613
TATAGTA605.410707E-428.7535615
TATCCCG756.146329E-527.6034185
GACTAAC500.007728426327.5994877
ATAAGAC906.8816244E-626.8366573
GTTATAT658.6172885E-426.5530911
CTTACAC951.0470538E-525.4242023
GACCGTG951.0482116E-525.420587
GTATAGA700.001327731524.6564431
AGACCGC700.001331042724.6459086
TCCCGGC700.00133214824.6423997
AGACACG851.4393045E-424.3559576
TATTATT1001.558153E-524.152992
CTATTGT1103.2554737E-521.9572644
TAGAGAT953.0571976E-421.7921715
GGGTACG953.0571976E-421.7921716
Produced by FastQC (version 0.11.2)