FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5333147
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA16100.016.9362241
GTATAGG7800.016.3727841
TAGCCTA6550.016.32781610
TAGGCCG3651.8189894E-1216.072295
GTATAAG13150.014.9611341
TAGGACA12850.014.7700274
CTATACT12750.014.6152184
TATATAC11500.014.4032693
TACACCG17000.013.8032615
TATACTG17450.013.6450595
GTATACA19000.013.6245781
GTATATA12000.013.518611
AGCCTAT7700.013.44107911
ACGGCCC13300.012.96958510
TACGGCC13850.012.7036389
CTTACAC17300.012.5664363
GTATAAC22700.012.4681891
GGCCGCG4205.806214E-912.3211067
GTATATG12700.012.2299381
GTGTAAG17000.012.1819761
Produced by FastQC (version 0.11.2)