FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5541229
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC7350.016.4317743
CCGTGCG1808.095766E-515.33300210
GCGACTG12450.015.24049816
TATACTG23800.014.4986255
GTATATA23950.014.4110731
TAGGACA15300.013.3065164
GTATTAG15250.013.1268321
ACCGAGC5850.012.97407910
CGACTGT14900.012.96604517
GTGCGAT12050.012.88353911
TTAGGAC11950.012.7054063
GCCCCGA4104.0308805E-912.6272631
TAGCCTA9300.012.61263110
TACGGCC12350.012.5705799
GTATAGA21300.012.4770781
AGGACAG17950.012.3032345
TTATACT26700.012.2776754
GGACAGG12100.012.2627236
ACGGCCC12150.012.20961310
CGGCCCA12900.012.03462511
Produced by FastQC (version 0.11.2)