FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6373976
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGAT77950.018.3668969
GGCATGA94150.015.86613868
GATCTCG21500.015.40410369
ACTAGGC218050.015.12535169
CATGATC49300.015.04510469
CTAGGCA170650.014.77796869
GACTAGG276350.014.36876769
ACCCCCC9900.013.9390088
TAGGCAT137050.013.84481969
GTATAGA15550.013.5386971
AGGCATG118750.013.39278769
CCCGCCT6350.013.03890617
GTATTAG11400.013.0179031
AGACTAG338300.012.777769
TGCGCGG4053.3796823E-912.77742310
TATAGGG7350.012.6761012
CTAGCAC18850.012.4476213
CTTACAC14450.012.4172173
TAGAGCG11450.012.3557925
CCCCCCA12050.012.3108629
Produced by FastQC (version 0.11.2)