FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422543
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT200.007353435751.7542633
AAGGGGG851.8189894E-1244.6454569
TCCTAGG350.001345523339.4364852
CCTAGGT400.002591932234.5069243
CCGGCTT450.004620230330.66556413
CGAGCAC450.004620230330.66556436
AAAGGGG1353.0559022E-1028.11009868
CCCGAGC500.00772636927.60227234
CTGTGCG500.007730833327.59900527
GCCGTCT1404.656613E-1027.10616746
GTCGACG658.6498144E-426.53750611
ATGCCGT1457.003109E-1026.17147344
TCTCGTA1359.39508E-925.55463638
CGTATGC1651.1459633E-1025.09000641
CTCCTAT3700.023.3099769
TAGACCG750.00198862623.0046165
CTCGTAT1653.1304808E-922.99917239
TATGCCG1756.175469E-921.68493543
CTTAACC800.002895047721.56427433
GTATGCC1959.295036E-1021.23000342
Produced by FastQC (version 0.11.2)