FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524262
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA70410.15562759185918057No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT60150.13294986010978144No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC49070.10845967806462137No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC46930.10372962485373305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTG5350.037.4136546
CGGGTGA5950.033.6285827
AGGGGGC16200.022.78615854
CAAGGGG17150.021.52371252
GATAGGC17850.020.8805276
GGGGCTT18300.020.17135256
TATACGG18950.019.8505825
ATAGGCC18600.019.8457897
GGGCTTT19150.019.4561757
AGCGGGT10850.019.4024265
GGGGGCT19500.019.2838755
CGATAGG19200.019.2326225
GGATCGA19650.019.1495741
CGGGTAC6550.018.43440249
CGGGCGG4900.018.30521452
AGGCCAC20650.018.209759
CCACACT21150.018.10548412
CGGCCCG1402.0792693E-417.2554365
TCGATAG21650.017.0561834
GGCCACA22000.016.93552210
Produced by FastQC (version 0.11.2)