FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524998
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA46600.10298347093192085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG18100.021.9230465
CCGCGCG1150.001111153317.9994099
AGGGGGC7200.017.24943454
CAAGGGG7650.017.13669252
CATATAC32700.016.8831653
TCATATA29250.016.6340832
ATATACG25050.016.2538384
CCATCAC30400.016.1174533
GGGGGCT7600.015.88763755
CTGATCG5150.015.4072619
ATACGGT28250.015.1455436
ATCACTG33600.014.685155
CGTAAGG15500.014.4706252
ACTGTAC34700.014.3163758
GTTCGGG5800.014.27570839
TACGGTG26350.014.1397977
CACTGTA33350.014.0683497
ACGGTGT29450.013.5885378
TATACTG21950.013.5190315
CATCACT34400.013.2402784
Produced by FastQC (version 0.11.2)