FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4880810
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG85450.17507339970209865No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT55750.11422284415906377No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA52590.10774850895650517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG12450.019.124835
GACCGAG30850.017.33319769
GTATATA21350.016.9766371
CATATAC19600.016.3736213
TCATATA19450.016.3244842
CTCGACT51100.016.20305625
ACTCGAC51600.015.77877724
CCGAGAT13150.015.74066912
TCGACTC53400.015.37596226
GTCGTGA13850.015.19497631
CATTCCC55600.014.95340112
CGTGAGC14800.014.91818312
AACTCGA56250.014.719727523
CGAGATC13850.014.44694213
GGCGTCG15150.014.34656232
TTATACT21450.014.317944
GTATTAA17400.014.2837921
TATATAC20400.014.2091123
GTATAGA14850.014.1795891
CCTCCGA13450.014.107119
Produced by FastQC (version 0.11.2)