FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966812
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA79890.20139598246652476No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT74170.18697634271551059No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC56990.14366700514166036No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG56170.14159985398854294No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC54240.13673448603059585No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT47370.11941579283313652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCAC35250.021.9274373
CATCACT36700.021.5312084
ATCACTG37100.021.2060575
TCCATCA39150.019.8322332
CACTGTA39600.019.3403247
ACTGTAC42050.018.7877798
TCACTGT42550.018.2466016
GTATATA13400.017.7722261
TCTATAC10850.016.5376033
TATACGG9700.016.00815
TCATATA12550.015.9479622
CTGTACT48950.015.7870619
TATACTG14900.015.7478645
TTATACT12450.015.5209274
GTATTAG9150.015.4653711
GTATAGG10800.015.3396351
CTATACT8400.014.7884344
CTGATCG3756.730261E-1114.7195539
TACCCTA15050.014.67065151
CTAGGAC3605.7843863E-1014.3776453
Produced by FastQC (version 0.11.2)