FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4985733
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT78180.15680743433312613No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA77800.15604525954358164No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC57790.1159107397046733No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG56810.11394513103690068No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT54060.10842939242835506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTG8700.032.921786
CGGGTGA8950.032.378657
AGCGGGT12750.024.0881965
TGCGTGG9350.018.8178410
GTATAGA12750.018.408711
TATACGG11250.018.4044655
CACTGTA40450.018.1661787
CATCACT42600.017.9832344
CCATCAC43100.017.5344163
TCATATA15950.017.525332
ATCACTG44250.017.3126755
CCCCACG11500.016.7993587
ACTGTAC44900.016.2889088
CCCACGC12300.016.2676728
CGTGGGG8150.016.08559812
TCACTGT45100.016.06826
CCACGCA12300.015.98751459
CGCGCAT7850.015.82105240
CATATAC17250.015.8038333
GGATACG39100.015.7938319
Produced by FastQC (version 0.11.2)