FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526809
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA5750.030.598987
GCGGGTG6250.028.1569736
AGCGGGT8500.022.7334255
TATACGG10950.022.6889365
TGCGTGG12200.021.20831110
GTGGGGC11350.020.66891513
CGTGGGG11900.019.71362912
GCGTGGG11800.019.5883311
CCCCCTG5750.018.599389
GAGCCCC11800.018.4227435
CCCCTGA5650.018.31797410
GGGGGGC13550.017.0584748
TCATATA18500.016.4144342
GGGGCTC14200.016.27762810
GTCCTAA16050.016.127371
TAAGGGG15650.016.0954935
ATATACG15500.016.0286354
GGGCAAA15500.016.02527216
GGATACG38400.015.8119749
GCCCCCC11050.015.610347
Produced by FastQC (version 0.11.2)