FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107412
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC252.5459583E-455.2469671
TCACCTC200.00734333251.74580828
GTAGGGA200.00734333251.7458088
TGCGCAA200.00734333251.74580820
AAACCCC200.00734333251.74580861
TCACTCT200.00734333251.74580820
GGCGCCG200.00734333251.74580825
TGAGACC200.00734333251.74580867
TATAACC306.2594865E-446.0391432
CCCGCCA404.9740433E-543.1215132
ACCCGCC350.001342908639.42537731
CTAGATA400.002575138634.5293544
ATAAAGG501.8409995E-434.5293543
TGGCGCC400.002586939834.49720824
AGCAGCG601.332017E-534.4972087
ATATACT450.00458511130.6927624
GCATGAT1151.6680133E-929.99757269
CTATATA500.00767230827.6234842
GGCATGA2050.026.92464869
GTAGTGG700.001326620224.6408629
Produced by FastQC (version 0.11.2)