FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797top2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797top2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10466237
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65550.035.84183511
ATCGGGG47800.035.6411540
GATCGGA60350.035.217451
ACACGTC66950.035.12553413
ACGTCTG68100.034.62961215
CACGTCT67950.034.60893214
CGTCTGA68850.034.25172816
TCGGGGG22500.034.14256341
GTCACAC68900.034.0015929
TCCAGTC69950.033.8342125
CACACGT70400.033.5915112
GAATCGG54500.033.5587538
CGGGGGT20800.033.54728742
AATCGGG54450.033.51048739
AGCACAC70950.033.2956510
GAACTCC70900.033.2251121
GAGCACA71000.033.1171469
AGAGCAC71300.032.9461678
CAGTCAC71550.032.8639927
ACTCCAG73000.032.2393423
Produced by FastQC (version 0.11.2)