FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797red1-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797red1-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9995944
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG37300.034.8565611
ACACGTC39000.033.45015313
ATCGGGG22950.033.1800840
TATCGGG27550.032.6716139
ACGTCTG39850.032.5712115
CACGTCT40100.032.31328614
CACACGT40200.032.23274212
GAACTCC42050.030.70541421
AGCACAC42850.030.54341910
CACTAGC41950.030.46811931
CGTCTGA43200.030.09590516
GATCGGA39650.030.0197941
CAGTCAC43750.029.7648627
TCGGGGT13900.028.18510441
GAGCACA46800.028.1063659
TCCAGTC46450.027.98638325
ACTAGCT46700.027.65314132
CCAGTCA47100.027.64713726
TCGGAAG47500.027.6005153
AGAGCAC48450.027.4665728
Produced by FastQC (version 0.11.2)