FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797i3h34c-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797i3h34c-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12068295
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21100.013.34433111
GATCTCG21600.013.04294639
ACGTCTG23200.012.6105715
CGATCTC21300.012.502954538
ACACGTC23800.012.38503213
CACGATC21700.012.27014436
CATCACG24950.011.81696933
CACACGT24950.011.63784712
CACGTCT26450.011.22739914
TCCAGTC25850.010.97641825
CGTATGC26950.010.94514144
CGTCTGA26950.010.77405216
CTCGTAT26000.010.58303442
CAGTCAC26450.010.56150227
AGTCACA28500.010.18788228
ATCTCGT29250.010.15878640
GAGCACA30350.010.0012779
AGCACAC28850.09.98758710
GAACTCC28500.09.9556821
ACGATCT29950.09.77355837
Produced by FastQC (version 0.11.2)