FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797i3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797i3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11447823
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC782970.6839466333467944TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG111450.039.1399511
CACACGT114100.038.32714512
ACTGACC112700.038.2605832
ACACGTC114100.038.23074713
ACGTCTG114450.038.07572615
CGTCTGA115300.037.83285516
CACGTCT115150.037.82532514
AGCACAC115500.037.8016410
GATCGGA104850.037.6899531
GAGCACA117750.037.079319
CAGTCAC117350.036.9077427
CGTATGC110750.036.869944
GAACTCC119150.036.56915321
CCAGTCA119400.036.30964326
TCCAGTC119050.036.286925
AGTCACT121250.035.7931728
AGAGCAC122600.035.665988
ATCTCGT115300.035.58395440
CTGACCA121500.035.5826233
TCGGAAG123850.035.55763
Produced by FastQC (version 0.11.2)