FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797i0h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797i0h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888295
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC243410.22355198862631845TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC218510.20068339441574645TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC147490.1354573879565166TruSeq Adapter, Index 2 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG275750.041.88119511
ACACGTC277000.041.66856413
CACACGT278250.041.5128112
GATCGGA255250.041.4288641
ACGTCTG278400.041.364415
CACGTCT278900.041.3217914
CGTCTGA279750.041.15616216
AGCACAC282200.041.0213310
CAGTCAC279100.040.85643427
CCGATGT280050.040.7210233
GAGCACA284500.040.7128949
CGATGTA280750.040.62191434
AGAGCAC285500.040.608078
GAACTCC280650.040.53394321
GTCACCG282350.040.51989729
TCCAGTC281500.040.466825
CCAGTCA281850.040.4011326
AGTCACC283650.040.3328928
TCGGAAG287800.040.31673
TATCGGG118250.040.2607639
Produced by FastQC (version 0.11.2)