FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7797hop1-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7797hop1-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10415631
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC296030.28421705799677427TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG40300.035.47847428
CGATCAG40750.034.66300633
GCACACG41550.034.5201611
GTCACGA42500.033.4357229
ACACGTC44550.032.24556713
CACACGT44900.032.0432112
CACGATC44500.031.98410831
ACGTCTG45150.031.76863715
GATCTCG44750.030.98624839
CACGTCT46750.030.82253814
GATCGGA42450.030.734151
CGTCTGA46650.030.65208616
CGTATGC46900.030.46021844
CAGTCAC48050.029.80177727
AGCACAC48550.029.6290710
GAACTCC49550.029.03041321
CCAGTCA49500.028.92698726
TCCAGTC49050.028.87819925
GAGCACA51100.028.279519
ATCTCGT50050.027.83771940
Produced by FastQC (version 0.11.2)