FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah77973htop2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah77973htop2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231830
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC283500.2770765346961394TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC152460.1490056031032572TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA97100.039.065221
GCACACG106050.039.01861211
ACGTCTG107700.038.64589315
ACACGTC107800.038.58944713
CACGTCT107550.038.55661414
CGTCTGA108300.038.4109116
CACACGT108850.038.11615412
CAGTCAC110050.037.75680527
TCCAGTC111350.037.19547325
CCAGTCA112450.037.00802626
AGTCACC112300.037.00032428
AGCACAC112100.036.98550410
GAACTCC113150.036.75838521
GAGCACA113200.036.7426839
AGAGCAC113550.036.6870048
TCGGAAG114150.036.458483
ACTCCAG115350.036.00028223
GTCTGAA116250.035.9922617
GTCACCT116050.035.82471529
TGAACTC118650.035.05462320
Produced by FastQC (version 0.11.2)