FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah77970htop2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah77970htop2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8250414
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC61850.037.59273513
GCACACG62000.037.39534411
CACGTCT62200.037.3106914
GATCGGA57950.037.0938451
ACGTCTG62550.037.06675315
CGTCTGA64100.036.23863616
CACACGT64350.035.99551812
TCCAGTC64350.035.58272625
AGCACAC65550.035.56931310
CAGTCAC64950.035.49279427
GAACTCC64800.035.26773521
CCAGTCA65100.035.10542326
GAGCACA67000.034.7664879
TCGGAAG67450.034.5690353
AGTCACC68300.034.04199628
AGAGCAC68800.033.9523968
GTCTGAA68900.033.71402717
CCAGATC69000.033.38654333
CATCGGG34050.033.1599439
ACTCCAG69100.033.13673423
Produced by FastQC (version 0.11.2)