FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7669i3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7669i3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11405322
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC210190.1842911581102226TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC183470.16086349863686444TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG157350.040.2768311
CACACGT158100.040.1273312
ACGTCTG160200.039.57350515
GTCACAC159100.039.52824829
ACACGTC160650.039.4491413
GATCGGA145600.039.4121131
CGTCTGA161350.039.23623316
CACGTCT162350.039.09025614
CACACAG161500.039.03856731
GAGCACA163200.038.9774259
AGCACAC163450.038.9043510
CAGTCAC163200.038.69541527
GAACTCC163100.038.622721
TCCAGTC164600.038.270925
CCAGTCA164500.038.2675926
AGAGCAC166900.038.204928
AGTCACA166900.037.8112228
ACACAGT167800.037.601932
TCGGAAG170400.037.5128483
CACAGTG168050.037.48510733
Produced by FastQC (version 0.11.2)