FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7669ha-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7669ha-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12264848
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT422890.3447984027197076TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC65250.036.3093113
GCACACG65700.036.1607711
ACGTCTG67950.034.93144615
CACACGT68250.034.87445412
CACGTCT68750.034.58910814
GATCGGA63550.034.4484751
CGTCTGA69750.033.80880416
CTCGTAT65850.032.8256344
ATCTCGT68950.032.14763342
TCGGAAG77100.031.1831723
AGCACAC77300.031.10172810
TCTCGTA70200.030.97969643
GTCACAG76600.030.92914829
CAGTCAC76900.030.74977127
CACAGTC77750.030.53037631
GAGCACA78600.030.4191659
AGTCAAC78950.029.90450734
CCAGTCA78850.029.87638926
AGAGCAC80550.029.5185598
ATCGGAA81600.029.2479762
Produced by FastQC (version 0.11.2)