FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7606top2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7606top2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8182732
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC128360.1568669241519825TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG20300.032.5079429
CACGGCT21500.030.7969831
TCACGGC21750.030.44243430
GCACACG22650.030.30240611
AGTCACG22350.029.72251928
ACGTCTG24500.027.92481415
ACACGTC24600.027.54285813
CGGCTAC24550.027.3331433
CATCTCG24100.026.57616639
CACACGT25550.026.2604612
ACGGCTA25600.026.20948632
GATCGGA24100.026.1089941
CACGTCT26050.026.09436814
AGCACAC26200.025.85816410
CGTCTGA26650.025.34147516
TCCAGTC26700.025.04409225
GGCTACA27000.024.7720334
CAGTCAC26850.024.74063927
CCAGTCA27450.024.27984426
AGAGCAC29250.023.9887588
Produced by FastQC (version 0.11.2)