FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7606red1-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7606red1-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9370947
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14500.023.36390111
AGTCACG14950.022.95236228
ACACGTC16500.020.79845213
CGTTTCG16200.020.64418433
GTCACGT17150.020.3932429
CGGGGGT5650.019.87103344
ATCGGGG11650.019.6521842
CACACGT17400.019.59624112
TCGGGGG8350.019.5096143
GATCGGA17450.018.786611
CACGTCT18500.018.31224614
ACGTCTG19100.017.85216715
CGTCTGA19950.017.42207516
GTTTCGG19600.017.17622434
AGCACAC20150.016.91919910
CAGTCAC21100.016.47086127
AATCGGG14400.016.20422241
GAACTCC20750.016.00562721
CCAGTCA22800.015.43522326
CGGGGGG3550.014.88269544
Produced by FastQC (version 0.11.2)