FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7606i3h34c-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7606i3h34c-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11404373
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC14500.017.14601933
ACTTAGG16800.014.928332
GCACACG20400.012.94003211
TTAGGCA22050.012.07419534
CACACGT21800.011.80634812
ACACGTC22200.011.5936213
CACGTCT23000.011.47729714
TAGGCAT23000.011.09734835
ACGTCTG23750.010.83698415
CAGTCAC24350.010.65954827
CGTCTGA26650.010.31781216
GAACTCC26850.010.07603921
CACTTAG26950.09.95869631
AGCACAC28000.09.89812710
CCAGTCA27750.09.670156526
AGTCACT28350.09.62117628
TCCAGTC27500.09.51807325
GAGCACA29750.09.3898199
AGGCATC24200.09.00196736
GATCGGA27550.08.8651221
Produced by FastQC (version 0.11.2)