FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah7606hop1-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah7606hop1-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12848535
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG17350.022.06049228
ACGTGGC17650.021.31362732
CACGTGG18250.021.09409531
GCACACG18600.020.8154311
TCACGTG20050.019.85842330
CGTGGCC19500.019.1806933
ACACGTC19850.018.95074813
GTCACGT21500.018.10959229
CACGTCT22250.017.2032314
ACGTCTG23200.017.06764615
GATCGGA21200.017.0207311
CACACGT23400.016.54579412
GGCCTTA24650.015.7106736
CGTCTGA25200.015.53823916
CAGTCAC25100.015.51183827
AGCACAC26300.015.0541710
GTGGCCT26550.014.75114334
GAGCACA29450.013.7426099
GAACTCC29850.013.55818921
CCAGTCA28850.013.49493726
Produced by FastQC (version 0.11.2)