FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah5187top2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah5187top2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10265532
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT484070.4715488685827485TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT246150.2397829941984497TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA166650.040.848511
GCACACG179350.040.79490311
CGTCTGA179550.040.68641716
ACGTCTG179600.040.65257615
ACACGTC181950.040.2121513
GAACTCC181500.040.20862621
CACGTCT181600.040.2048614
CACACGT183150.040.03276412
AGTTCCG181000.039.97149734
CCGTATC178400.039.9100738
CAGTCAC182250.039.87769727
GAGCACA184550.039.7603769
AGCACAC185600.039.5591410
AGAGCAC186100.039.4509358
TCCAGTC184150.039.4398625
CCAGTCA184550.039.37898326
GTTCCGT185050.039.14538635
TCGGAAG188400.039.1354263
GTCACAG186450.039.0633229
ACTCCAG186450.039.01175723
Produced by FastQC (version 0.11.2)