FastQCFastQC Report
Thu 17 Aug 2017
HN2LKAFXX_n01_ah5187i3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2LKAFXX_n01_ah5187i3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10941360
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1385831.2665975710514965TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC185500.042.04014229
TCACGCC186600.041.74611730
GCACACG191100.041.28922711
AGTCACG190050.041.22854628
CACGCCA190800.040.79426631
ACGTCTG194000.040.63837415
CACACGT195000.040.56515512
ACACGTC194850.040.52864513
CGTCTGA194400.040.4864916
GATCGGA180500.040.4324071
TATCTCG181000.040.26121539
CACGTCT196450.040.17634614
AGCACAC197900.039.93426510
GAGCACA198150.039.8616839
GAACTCC195900.039.82530221
CAGTCAC197800.039.77926627
CGTATGC175100.039.6757144
CCAGTCA199500.039.37215426
TCCAGTC199550.039.35090625
AGAGCAC203050.038.9535458
Produced by FastQC (version 0.11.2)