FastQCFastQC Report
Mon 22 Jan 2024
HN2GGAFX5_n01_DAM-GR-GBF2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2GGAFX5_n01_DAM-GR-GBF2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10299043
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAT8189317.951525204817574TruSeq Adapter, Index 8 (97% over 36bp)
ATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGAA655060.6360396786381026Clontech Universal Primer Mix Short (100% over 22bp)
CTATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGG650600.6317091791926687Clontech Universal Primer Mix Short (100% over 22bp)
TATAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGA577570.5607996781836915Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGAGATCGG558580.5423610718005547Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCGCGTAT555950.5398074364773504TruSeq Adapter, Index 8 (97% over 36bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAAGATCGGA494480.48012227932245743Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTATAGATCGGAA458770.4454491548389496Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAAGATCGGA390520.3791808617557962Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGAGATCGG357470.34709050151552917Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGATCGGAA267430.2596649028458275Clontech Universal Primer Mix Short (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGGAT211730.20558220797796456TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATATCGTAT183440.178113636383497TruSeq Adapter, Index 8 (97% over 36bp)
TAGGGCAGCGTGGTCGCGGCCCTATAGTGAGTCGTATTAGAGATCGGAAG158400.1538006977929891Clontech Universal Primer Mix Long (95% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAA157130.1525675735114418TruSeq Adapter, Index 8 (97% over 36bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCAC131870.12804102284066587Clontech Universal Primer Mix Long (95% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTAGTAT127320.12362313663512232TruSeq Adapter, Index 8 (97% over 36bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTATAGGG125210.12157440259255156Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGAGATCGGAAGAGCAC121790.11825370570838474Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGACCACGCTGCCCTAAGATCGGAAG116470.11308817722190305Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)