FastQCFastQC Report
Fri 7 Feb 2025
HN277AFX7_n01_AH9643-0hr-Rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN277AFX7_n01_AH9643-0hr-Rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23254580
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT9765554.199409320658554TruSeq Adapter, Index 25 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)