FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15401411
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288828418.753372661764562No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC775870.5037655316126555Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313920.2038254806653754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG130000.061.78447718
AGAGCAC174150.059.87034615
AGCACAC135450.058.58699817
CACACGA73000.057.5091519
ACACGAG71500.056.90577720
GCACGCG26600.056.73158618
TAACTGA124100.055.411452
CGAGTCC15500.054.65279817
TCATACT99250.053.0076612
CACGTAG32300.052.81324821
CACACGT60100.052.1421819
ACTGAAG131200.052.0027244
CATACTT99450.051.8697113
CACGCGT11350.051.82676319
CACACGG12450.050.62252419
AGATCAT135500.050.1004189
ACGAGAT85100.050.052722
ACGAGTC17500.049.76702516
GAGCACA190900.049.6711216
GCGCACG23500.049.3161818
Produced by FastQC (version 0.11.5)