FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9620552
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1292931.3439249639729611No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1181981.2285989411002611Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA278850.035.24341247
GGTCGCC286700.034.70568544
GTCGCCG284350.034.6232645
TCGCCGT284650.034.51346
CCGTATC282400.034.4907249
GCCGTAT288000.034.02665348
CGTATCA292150.033.4479950
GTGGTCG297500.033.34042442
CTCGGTG293350.033.07271638
TGGTCGC302700.032.81358743
AAAGGGG181050.032.49564766
ATCTCGG301300.032.3165736
TCTCGGT302500.032.1305237
GGTGGTC311400.032.02112641
GATCTCG301800.031.78755235
TCGGTGG312200.031.6365539
AAGGGGG187450.031.36749567
CGGTGGT321350.031.09500140
GTATCAT310050.031.06525651
AGATCTC315150.030.66296434
Produced by FastQC (version 0.11.5)