FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667027
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5398744.627348509607461Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1853061.5882880874450704No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC136600.11708209812148374Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA258400.052.0510416
AGAGCAC270050.051.82776315
AAGAGCA495650.050.99617814
GAGCAAG173500.049.68399416
CACACGA79550.048.92313419
ACACGAG80500.048.47579220
GCACAAG120400.048.1086418
AGAGCAA192700.046.35047515
CGAGTCC21000.046.1651117
AGCACAA128300.045.66486717
CACGAGA87050.045.47194321
GCACACG112300.045.31439218
GTGACTG68500.044.9148521
AGCACAC140950.044.29790517
TGACTGG75900.042.1598742
AGCAAGA209250.041.48008317
ACGAGTC24550.039.48933416
AGTCCTC37250.039.36746219
GACTGGA84500.038.937773
CACAAGA149100.038.9185419
Produced by FastQC (version 0.11.5)