FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7672088
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1052591.371973314174707No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC405430.5284480574258272Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA79750.043.62217347
GGTCGCC82750.042.54823344
GTCGCCG84600.041.3282245
TCGCCGT84450.041.27729846
CCGTATC83550.041.13548349
CTCGGTG87150.039.3567138
GTGGTCG89750.039.1129842
GCCGTAT90450.038.26831448
CGTATCA90900.037.96360450
ATCTCGG91950.037.37881536
TCTCGGT92100.037.2796937
TGGTCGC97050.036.09881643
GATCTCG94800.035.77582635
TCGGTGG99150.035.33470539
GGTGGTC102200.034.45142441
AGATCTC101600.033.69076534
CGTGTAG108800.033.19705616
AGCGTCG113000.032.70693611
GCGTCGT113400.032.5915712
CGGTGGT109450.032.26528540