FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_548-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_548-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922703
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1251711.579902717544757No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC200280.252792512858301Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA45700.036.91343747
GGTCGCC46800.036.04558644
GTCGCCG47650.035.4762645
TCGCCGT48850.034.3898546
CCGTATC51850.032.73758349
CTCGGTG51750.032.5313538
ATCTCGG53600.031.34323336
GTGGTCG54450.031.17455342
GCCGTAT57500.029.8249148
TCTCGGT58500.028.89738837
TGGTCGC58500.028.71717643
CGTGTAG62100.028.51757216
GATCTCG58400.028.10800735
AGCGTCG66150.027.82976511
CGTATCA62150.027.36835950
GTCGTGT66800.027.13981814
CGTCGTG67350.027.12604913
GCGTCGT68000.026.91822412
TCGGTGG65750.026.66882139
GTGTAGG67150.026.21655317
Produced by FastQC (version 0.11.5)