FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11033714
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1376921.2479206910746463No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC469460.42547776750421484Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTC10100.027.7603821
CCGGGTG10700.026.858821
CGGGGAG13300.026.6150051
CGGGGTT20750.024.6599161
CGGGGAC11250.023.3649861
CGGGGAT18100.023.0422671
CGGGGTG16750.023.0162561
GAGGGTA37350.021.7697891
AAAGGGG107300.021.69102166
GGGGGAT30700.021.6906241
GGGGTTA31350.021.6664432
GCGGGTC11200.021.5917531
GAGGGTC19750.020.939761
GGGCGTC13000.020.7588941
AGAGCAC59500.020.35229115
GAGCACA56600.019.91093416
AAGGGGG116850.019.79843567
GCGGGTG20100.019.354581
CCGTATC217200.019.20757749
CGCCGTA218050.018.90781647
Produced by FastQC (version 0.11.5)