FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1664-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1664-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11833042
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1809601.5292770869908177No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC298100.2519216951989184Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC12100.028.386061
CGGGGTT21250.027.7086121
CGGGGAG15700.027.0115911
CCGGGTC9750.026.2411381
CCGGGTG11650.024.9698871
CGGGGTG17100.022.9554751
AAAGGGG85400.021.18805166
GAGGGTA39800.020.7822881
GAGGGTC21250.020.7814581
GGGGGAT32500.019.8426131
GGCGGTC11700.019.4711631
AAGGGGG95250.019.32765467
GGGGTTA34650.019.3002472
GGCGGTA33300.019.1554151
CGGCGTG9500.018.4463651
AGAGCAC48950.018.37517415
GGGGAAC23700.018.3191812
GGGGTAA36750.018.1973742
GAGGGTT42600.018.1822281
GAGCACA48400.018.07780516
Produced by FastQC (version 0.11.5)