FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667868
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1622221.390331121332535No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1046310.8967448037636353Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTT20700.033.3475881
AGGGGTA38250.029.7728561
GAGCAAG55650.029.49619316
GGGGGAT42900.029.4861771
GCGGGTC21300.028.9535391
CACACGA23150.028.87574819
AAGAGCA151900.028.82411614
AAGGGGG191850.028.55055667
AAAGGGG198450.027.8479466
GGGCGTA20900.027.6634451
GAGGGTT41950.027.6480561
GAGGGTA38650.027.1982121
GGGGTCA23350.026.690412
GGGGTAA46100.026.2023532
CACGAGA25050.025.98677421
GGGGTAC18750.025.9558752
GGGCGTT20550.025.5769061
GGCGGTC14950.025.5478251
GCGGGTA25000.025.3692231
GCACACG34450.025.29685818
Produced by FastQC (version 0.11.5)