FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1644-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1644-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10958032
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1517911.3852031094634512No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1151661.0509733864620947Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAG58000.033.12805616
AAGAGCA155450.032.91624514
GAGCACA80450.030.84407816
AAGGGGG197500.030.51627767
AGAGCAC86450.030.48495515
AAAGGGG199950.030.36991566
GCGGGTT19250.030.0339531
CACACGA20950.027.7316519
GCGGGTA24550.027.2609581
GGGGGAT34600.026.8366951
AGAGCAA74350.026.69055715
GCGGGTC19250.026.5754971
AGGGGGG234250.026.37136568
AGGGGTA35400.026.3291991
GGGGTCA22550.026.2375072
GCACACG30450.026.20590618
GGGCGTA17600.026.0806181
GAGGGTT38850.025.7047331
AGGGGTC20750.025.6675681
CGTATCA400500.025.44746250
Produced by FastQC (version 0.11.5)