FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8993223
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1160871.2908275486997265No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC601390.6687146532450046Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA141850.034.41910647
GGTCGCC145600.034.15760444
GTCGCCG146000.033.89621745
TCGCCGT144100.033.78452746
CCGTATC144250.033.5554849
GCCGTAT149750.032.67363748
AAAGGGG111450.032.47132566
GTGGTCG155950.031.84594342
TGGTCGC157250.031.42669943
CTCGGTG154800.031.2237238
AAGGGGG115900.031.07359167
CGTATCA157250.030.82610350
ATCTCGG159200.030.49266436
TCTCGGT159250.030.43913537
GGTGGTC166950.029.8105741
GATCTCG161850.029.64773635
TCGGTGG170450.028.95204439
AGATCTC170400.028.38606534
CGGTGGT177900.028.09372940
CGTGTAG187800.028.01032316
Produced by FastQC (version 0.11.5)