FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10104203
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4521194.474563703836909Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1627841.6110523511849475No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC115140.11395257993134145Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC193100.049.1901915
GAGCACA185100.048.55537816
AAGAGCA351300.047.39209714
CACACGA62100.046.72095519
ACACGAG62450.046.40260320
GCACACG84450.045.46275318
GAGCAAG125250.044.45710816
CACGAGA68350.043.37042221
AGCACAC109800.042.20213317
GCACAAG85450.041.1216418
AGAGCAA140250.040.97521215
GTGACTG43700.038.8909761
GATCGAG27850.038.3297778
AGCACAA93600.037.6906317
CGATCGG15350.036.5258331
AGCAAGA153950.036.4420317
AAGGGGG615300.035.78481367
AAAGGGG616700.035.71492466
TGACTGG50600.034.8031732
GCAAGAT160100.034.7579818
Produced by FastQC (version 0.11.5)