FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9037236
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1176211.3015151977883503No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC591390.6543925598490512Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA140050.033.46195647
GGTCGCC142950.033.10140244
GTCGCCG145250.032.1676245
TCGCCGT145650.031.98316446
GCCGTAT147950.031.91194348
CCGTATC147550.031.8798549
GTGGTCG149750.031.3644142
CGTATCA153950.030.73641650
CTCGGTG151000.030.41044238
ATCTCGG156400.029.71868536
TCTCGGT157350.029.3613137
GGTGGTC160950.029.18185841
GATCTCG157200.029.09988635
TCGGTGG162650.028.76942639
TGGTCGC168950.027.92404643
CGGTGGT169650.027.85039340
AGATCTC167700.027.82067934
AGCGTCG190150.027.73725911
GCGTCGT191000.027.61397412
CGTGTAG188100.027.5187316