FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933159
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1000151.1195927442912412No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC794790.8897076610860727Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA202800.031.44397547
GGTCGCC207000.031.3470344
CCGTATC206000.030.97268949
TCGCCGT207050.030.73092846
GTCGCCG209600.030.62422445
GCCGTAT209350.030.49378448
CGTATCA213600.030.00174750
GTGGTCG215500.029.89913742
CTCGGTG215400.029.11783438
AAAGGGG134950.028.83973966
GGTGGTC226350.028.4971841
ATCTCGG223100.028.3481936
TCTCGGT222200.028.33737
TGGTCGC229250.028.16690843
GATCTCG222000.028.01599535
TCGGTGG229550.027.749439
GTATCAT229250.027.64799951
CGGTGGT235150.027.52003540
AAGGGGG144850.026.9169767
AGATCTC235900.026.86935634
Produced by FastQC (version 0.11.5)