FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10257097
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2778752.709099855446429Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1623351.5826602790243671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA88900.045.98240316
AGAGCAC93000.045.1222815
CATTAAA630550.038.13277455
CACACGA27900.037.75797319
GTATCAT612200.037.72571651
AAAGGGG373250.037.58297766
GCACACG36400.037.2098618
AAGGGGG382000.036.65797467
CGTATCA644050.036.5665250
CCGTATC642750.036.50979649
ATCATTA648850.036.45854653
AAGAGCA190800.036.3196514
TCATTAA653550.036.18564254
TATCATT660400.035.6246452
ATTAAAA706250.035.20510556
AGCACAC51000.035.20429617
ACACGAG29600.034.87984520
GCCGTAT678700.034.77702348
TTAAAAA720900.034.6013457
AGGGGGG407400.034.57899568
Produced by FastQC (version 0.11.5)