FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n02_1215a-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n02_1215a-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11317546
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3155472.788122089364603Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1735461.5334242953375228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC131550.042.94013215
GAGCACA125050.042.8489616
AAGAGCA263550.041.3794914
GAGCAAG105550.038.43031716
GCACAAG77250.037.42228718
AAGGGGG444250.035.57874367
AAAGGGG448250.035.39414666
ACACGAG33700.034.68642820
CACACGA34600.033.78433219
AGGGGGG479250.033.22154668
AGCACAA88750.033.0464217
AGAGCAA126350.032.9903715
CGATCGG13300.032.673151
GCACACG45300.032.3716318
AAAAGGG508300.031.5498465
AGCACAC64700.030.23822617
AGCAAGA135900.030.07960717
GCAAGAT135550.030.05399718
CACAAGA103600.027.90392119
CACGAGA41750.027.83073221
Produced by FastQC (version 0.11.5)