FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15401411
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA917080.5954519361894829TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597620.38802938250268104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG91250.049.859581
TGACTGG96650.047.4627652
ACTGGAG113250.041.1513674
GGAGTTC120850.038.896947
GACTGGA139150.033.5429043
GAGTTCA140650.033.197928
TGGAGTT144650.032.5324556
CTGGAGT154050.030.5473545
TCACAGA249100.030.06034731
AGCTCGG42650.028.81220441
AAAAAGG306300.027.92898670
CACAGAT273900.027.32886732
GCTCGGA35300.026.40541342
AGTTCAG178100.026.2760079
GCTTGAA374150.023.75799660
GTCACAG354050.022.95719330
ACAGATC333300.022.35641533
GGAGCGC11350.021.93885447
CTTGAAA419450.021.69265261
AGCGCCC26000.020.77474449
Produced by FastQC (version 0.11.5)