FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9620552
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG885650.9205812722596374TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG98340.10221866687067437TruSeq Adapter, Index 10 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT201150.032.6410350
CTGCTTG211900.032.37249858
TGCCGTC222700.032.0282249
TATGCCG238200.031.67786247
AAAAAGG252250.031.28702270
GTATGCC249100.031.2611646
ATGCCGT228550.031.07059948
GCTTGAA236550.030.81876260
CCGTCTT222250.030.62872551
CGTATGC251050.030.3073645
TGCTTGA228900.030.19744159
TATCTCG263350.029.835440
CTCGTAT243650.029.3604943
ATCTCGT259650.029.2226641
TCGTATG240550.028.85135344
TCTCGTA255750.028.69667842
AGATCGG374200.028.3589571
GCTTATC343050.028.04568937
GATCGGA377700.028.034262
CACTAGC356950.028.01248632
Produced by FastQC (version 0.11.5)