FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667027
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5531584.741207850123258TruSeq Adapter, Index 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG534640.4582487038043197No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA270540.23188426666022116TruSeq Adapter, Index 14 (97% over 38bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209900.1799087291046811No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC174510.1495753802575412TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG154040.13203020786700845TruSeq Adapter, Index 1 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT131140.11240224266216235TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATG122110.10466248171020776TruSeq Adapter, Index 1 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG713700.052.52965570
AAAAAAG904300.042.25520769
AGGGGGG35500.042.2232631
CGTGTGC140800.042.160281
GTGTGCT166600.035.7500232
CCTACAC80550.035.3678411
ACGACGC80300.035.34719516
CTACACG80900.035.3013512
CGACGCT80750.035.10687317
ACGTGTG58950.034.6948851
CCCTACA83050.034.42960710
TCCCTAC83700.034.329499
ATGCCGG31100.034.09823670
ACACGAC86050.033.71734214
CACGACG86450.032.9540715
TCGGGGG8050.032.18694
GACGCTC87300.032.03186418
CTTGAAA1432300.031.96122661
TGAAAAA1461200.031.69555363
TTGAAAA1456950.031.49254262
Produced by FastQC (version 0.11.5)