FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7672088
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG347760.45327947228968174TruSeq Adapter, Index 16 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG77150.040.1473258
GCCGTCT78050.039.37074750
TGCCGTC82000.039.30927749
TATGCCG86050.038.0285747
ATGCCGT87150.037.22730348
CATCTCG80000.036.92340540
CCGTCTT85950.036.19994751
TGCTTGA85500.036.1446359
GTATGCC92650.035.73509646
ACCCGTC99000.035.38804633
TCACCCG99300.035.3516331
CTCGTAT87950.034.81941643
CCGTCCA99700.034.64767535
CACCCGT101500.034.51642232
GTCCATC91550.033.90896237
GTCACCC104500.033.7264730
CCCGTCC103850.033.70121834
GCTTGAA96900.033.44540460
CGTCCAT98100.033.28602236
CGTATGC100250.032.81653245